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Kinetics of Activity-Evoked pH Transients and Extracellular pH Buffering in Rat Hippocampal Slices

¹Department of Physiology and Neuroscience and Department of Neurosurgery, New York University School of Medicine, New York, New York; and ²Department of Chemical and Biomedical Engineering, Florida State University, Tallahassee, Florida

Submitted 14 December 2005; accepted in final form 27 February 2006

The kinetics of activity-dependent, extracellular alkaline transients, and the buffering of extracellular pH (pH_e), were studied in rat hippocampal slices using a fluorescein-dextran probe. Orthodromic stimuli generated alkaline transients 0.05 pH units that peaked in 273 ± 26 ms and decayed with a half-time of 508 ± 43 ms. Inhibition of extracellular carbonic anhydrase (ECA) with benzolamide increased the rate of rise by 25%, doubled peak amplitude, and prolonged the decay three- to fourfold. The slow decay in benzolamide allowed marked temporal summation, resulting in a severalfold increase in amplitude during long stimulus trains. Addition of exogenous carbonic anhydrase reduced the rate of rise, halved the peak amplitude, but had no effect on the normalized decay. A simulation of extracellular buffering kinetics generated recoveries from a base load consistent with the observed decay of the alkaline transient in the presence of benzolamide. Under control conditions, the model approximated the observed decays with an acceleration of the CO₂ hydration–dehydration reactions by a factor of 2.5. These data suggest low endogenous ECA activity, insufficient to maintain equilibrium during the alkaline transients. Disequilibrium implies a time-dependent buffering capacity, with a CO₂/HCO₃^– contribution that is small shortly after a base load. It is suggested that within 100 ms, extracellular buffering capacity is about 1% of the value at equilibrium and is provided mainly by phosphate. Accordingly, in the time frame of synaptic transmission, small base loads would generate relatively large changes in interstitial pH.

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