Journal of Neurophysiology

A chamber for the perfusion of in vitro tissue with multiple solutions

Matthew G. Thomas, James A. Covington, Mark J. Wall

Abstract

There are currently no practical systems that allow extended regions (>5 mm2) of a tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to pharmacological manipulation. Previous work has only achieved this at the expense of access to the tissue for recording electrodes. Here, we present a chamber that allows a tissue slice to be maintained in multiple solutions, at physiological temperatures, and preserves the ability to record from the slice. We demonstrate the effectiveness of the tissue bath with respect to minimizing the mixing of the solutions, maintaining the viability of the tissue, and preserving the ability to record from the slice simultaneously.

  • tissue perfusion
  • microstereolithography
  • seizure generalization
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