CA1 inhibitory interneurons at the stratum lacunosum-moleculare and radiatum junction (LM/RAD-INs) display subthreshold membrane potential oscillations (MPOs) involving voltage-dependent Na+ and A-type K+ currents. LM/RAD-INs also express other voltage-gated K+ currents, however their properties and role in MPOs remain unclear. Here, we characterized these voltage-gated K+ currents and investigated their role in MPOs. Using outside-out patch recordings from LM/RAD-IN somata, we distinguished four voltage-gated K+ currents based on their pharmacology and activation/inactivation properties: a fast delayed rectifier current (IKfast), a slow delayed rectifier current (IKslow), a rapidly inactivating A-type current (IA), and a slowly inactivating current (ID). Their relative contribution to the total K+ current was IA > IKfast > IKslow = ID. The presence of ID and the relative contributions of K+ currents in LM/RAD-INs are different from that of other CA1 interneurons, suggesting the presence of differential complement of K+ currents in subgroups of interneurons. We next determined if these K+ currents were sufficient for MPO generation using a single-compartment model of LM/RAD-INs. The model captured the subthreshold voltage dependence of MPOs. Moreover, all K+ currents were active at subthreshold potentials but ID, IA and the persistent sodium current (INaP) were most active near threshold. Using impedance analysis, we found that IA and INaP contribute to MPO generation by modulating peak spectral frequency during MPOs and governing the voltage range over which MPOs occur. Our findings uncover a differential expression of a complement of K+ channels that underlies intrinsic rhythmic activity in inhibitory interneurons.
- Outside-out patch recording
- theta rhythmic activity
- single-compartment mathematical model
- Copyright © 2009, Journal of Neurophysiology