Table 1.

Morphological and electrophysiological parameter comparisons between the 5-HT3A KO and WT neurons

ParametersWTKO
Total dendritic length, μm1,095.3 ± 63.61,476.0 ± 69.2
No. of endpoints15.6 ± 0.9120,6 ± 0.99
Sum of branch order83.8 ± 6.8112.2 ± 8.2*
Input resistance, MΩ128.5 ± 12.0118.4 ± 11.0
Membrane time constant, ms14.9 ± 1.218.9 ± 1.1*
Cellular capacitance, pF120 ± 0.6180 ± 1.2
Resting potential, mV−58.2 ± 1.9−66.9 ± 2.5
First AP amplitude, mV82.4 ± 3.492.6 ± 1.6
Sequential AP amplitude, mV71 ± 2.880.5 ± 1.7
First AP half-width, ms1.15 ± 0.0640.92 ± 0.027
Sequential AP half-width, ms1.88 ± 0.161.38 ± 0.067
AP threshold, mV−30.87 ± 0.91−37.38 ± 0.80
Rheobase, pA170.95 ± 16.85146.67 ± 11.56
AHP amplitude, mV10.3 ± 0.4810.1 ± 0.53
AHP slope, mV/ms0.19 ± 0.010.26 ± 0.016
Normalized AHP slope0.79 ± 0.0240.87 ± 0.013
Normalized AHP amplitude0.975 ± 0.0170.959 ± 0.012
  • Values are means ± SE for wild-type (WT; n = 21) and 5-HT3A receptor knockout (KO; n = 24) neurons. AP, action potential; AHP, afterhyperpolarization.

  • * P < 0.05 and †P < 0.01.